Updated Ground Truth Checklist

-Use 1 segment for all extracellular space.

-Use 1 segment for objects that connect 20 slices outside the boundaries.

-Parallel plane boundaries should be given the area they need. Careful not to over color them and allow space for the boundary.

-When segmenting thin lines of glial or extracellular space it's ok to bend the parallel boundary rule and segment darker than normal areas. (so long as you believe they are part of those objects)

-Be cognizant of invaginations and be careful of internal objects such as organelles/ER always use a keen eye when investigating them.

-Keep an eye on the borders of your volume and always check for small segments that just barely pass by. These are important and should be segmented.

-For the dust/defects/fat globules:

-If dust/defects are (nearly) fully contained within a single cell, then color over them.

-If dust/defects span multiple cells:

          -If they are small enough that it's easy to draw an imaginary boundary between the involved cells, then do so.

          -If they are large enough that drawing an imaginary boundary between the involved cells is not easy, then we should create a separate "defect mask" that can "safely" cover the dust or defects.

-For the fat globules (or ER-membrane swirling, whichever applies), we should color them separately and as accurately as possible.

-Myelin should be its own segment.

-Always check with the project leader when you have questions and keep a collection of images to help illustrate tough areas you want clarification for.

-Be mindful that these rules change and it’s always a good idea to ask for help if you aren’t sure about something.